Inhibition and Activation of Polynucleotide Phosphorylase by Acridine Orange
نویسندگان
چکیده
منابع مشابه
Inhibition and activation of polynucleotide phosphorylase by acridine orange.
Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...
متن کاملInhibition and Activation of Polynucleotide Phosphorylase by A&dine Orange ++
Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...
متن کاملThe inhibition of polynucleotide phosphorylase by specific polymers.
Mii and Ochoa (7) discovered that the synthesis of certain homopolymers by polynucleotide phosphorylase is stimulated by the corresponding polynucleotides or by various copolymers, as well as by ribonucleic acid. These results were confirmed by Singer, Heppel, and Hilmoe (8, 9), who also showed that oligonucleotides are good primers for polynucleotide synthesis. In addition to these stimulation...
متن کاملPolynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
متن کاملCarotenoid pigments and photokilling by acridine orange.
It has long been known that certain organic dyes can photosensitize cells, leading to cellular damage or death. Recently, studies were performed on one such dye, Toluidine Blue, to determine the mechanism of its lethal photosensitization of bacteria (M. M. Mathews, J. Bacteriol. 85:322, 1963). These studies showed that the cellular site of the lethal action was the cytoplasmic membrane and its ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)67930-5